Sheep NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Produktübersicht

• Artikelname: Sheep NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
• Artikelnummer: EBT-ELK0360
• Hersteller Artikelnummer: ELK0360
• Alternativnummer: EBT-ELK0360-96, EBT-ELK0360-48, EBT-ELK0360-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Sheep
• Alternative Synonym: NT-Pro-BNP, N-BNP, ANFB_HUMAN, BNP(1-32), BNP(5-29), BNP-32, Gamma-brain natriuretic peptide, nppb

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.067 ng/mL
• Proben: Serum, plasma and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Endocrinology,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Sheep NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve