Human ERK1/2(Extracellular Signal Regulated Kinase 1/2) ELISA Kit

Produktübersicht

• Artikelname: Human ERK1/2(Extracellular Signal Regulated Kinase 1/2) ELISA Kit
• Artikelnummer: EBT-ELK0372
• Hersteller Artikelnummer: ELK0372
• Alternativnummer: EBT-ELK0372-96, EBT-ELK0372-48, EBT-ELK0372-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human
• Alternative Synonym: ERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK

Produkteigenschaften

• Konzentration: 20 ng/mL
• Detektionsbereich: 0.32-20 ng/mL
• Sensitivitaet: 0.12 ng/mL
• Proben: tissue homogenates, cell lysates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Enzyme & Kinase,Apoptosis,Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ERK1/2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ERK1/2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ERK1/2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human ERK1/2 in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK0372