Human HIF3alpha(Hypoxia Inducible Factor 3 Alpha) ELISA Kit

Produktübersicht

• Artikelname: Human HIF3alpha(Hypoxia Inducible Factor 3 Alpha) ELISA Kit
• Artikelnummer: EBT-ELK0433
• Hersteller Artikelnummer: ELK0433
• Alternativnummer: EBT-ELK0433-96, EBT-ELK0433-48, EBT-ELK0433-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human
• Alternative Synonym: Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17, bHLHe17, HIF3-alpha-1, Inhibitory PAS domain protein, IPAS, Member of PAS protein 7, PAS domain-containing protein 7

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.056 ng/mL
• UniProt: Q9Y2N7
• Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HIF3alpha. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HIF3alpha. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HIF3alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human HIF3alpha in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK0433