Human ABAT(4-Aminobutyrate Aminotransferase) ELISA Kit

Artikelnummer: EBT-ELK0500
Artikelname: Human ABAT(4-Aminobutyrate Aminotransferase) ELISA Kit
Artikelnummer: EBT-ELK0500
Hersteller Artikelnummer: ELK0500
Alternativnummer: EBT-ELK0500-96, EBT-ELK0500-48, EBT-ELK0500-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: GABAT, NPD009, GABA-AT
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.15 ng/mL
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ABAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ABAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ABAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human ABAT in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0500