EasyStep Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

Produktübersicht

• Artikelname: EasyStep Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
• Artikelnummer: EBT-ELK053ES
• Hersteller Artikelnummer: ELK053ES
• Alternativnummer: EBT-ELK053ES-96, EBT-ELK053ES-48, EBT-ELK053ES-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Rat
• Alternative Synonym: 25 Hydroxy Vitamin D,

Produkteigenschaften

• Konzentration: 5000 ng/mL
• Detektionsbereich: 78.13-5000 ng/mL
• Sensitivitaet: 7.7 ng/mL
• Proben: Serum, plasma

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Competitive Inhibition. Assay length: 1.5h. Research Area: Reproductive science,Genetic science,Nutrition metabolism,Bone metabolism,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D antigen, and the Rat 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK053ES