Horse MCP1(Monocyte Chemotactic Protein 1) ELISA Kit

Produktübersicht

• Artikelname: Horse MCP1(Monocyte Chemotactic Protein 1) ELISA Kit
• Artikelnummer: EBT-ELK0634
• Hersteller Artikelnummer: ELK0634
• Alternativnummer: EBT-ELK0634-96, EBT-ELK0634-48, EBT-ELK0634-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Equine
• Alternative Synonym: CCL2, GDCF2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF, Chemokine C-C-Motif Ligand 2, Monocyte Chemotactic And Activating Factor, Monocyte Secretory Protein JE

Produkteigenschaften

• Konzentration: 2000 pg/mL
• Detektionsbereich: 31.25-2000 pg/mL
• Sensitivitaet: 13.4 pg/mL
• Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse MCP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse MCP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse MCP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Horse MCP1 in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK0634