Hamster ADIPOQ(Adiponectin) ELISA Kit

Artikelnummer: EBT-ELK0660
Artikelname: Hamster ADIPOQ(Adiponectin) ELISA Kit
Artikelnummer: EBT-ELK0660
Hersteller Artikelnummer: ELK0660
Alternativnummer: EBT-ELK0660-96, EBT-ELK0660-48, EBT-ELK0660-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Hamster
Alternative Synonym: ADP, GBP28, ApM1, AdipoQ, Acrp30, ACDC, APM1, C1Q And Collagen Domain Containing, Adipocyte Complement-Related Protein Of 30 KDa, Adipose Most Abundant Gene Transcript 1
Konzentration: 30 ng/mL
Detektionsbereich: 0.47-30 ng/mL
Sensitivitaet: 0.16 ng/mL
Proben: Serum, plasma and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,Infection immunity,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster ADIPOQ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster ADIPOQ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster ADIPOQ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Hamster ADIPOQ in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0660