Human Anti-GM1(Anti-Ganglioside Antibody) ELISA Kit

Artikelnummer: EBT-ELK0676
Artikelname: Human Anti-GM1(Anti-Ganglioside Antibody) ELISA Kit
Artikelnummer: EBT-ELK0676
Hersteller Artikelnummer: ELK0676
Alternativnummer: EBT-ELK0676-96, EBT-ELK0676-48, EBT-ELK0676-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: anti-ganglioside (GM1) antibody (IgG)
Konzentration: 80 ng/mL
Detektionsbereich: 1.25-80 ng/mL
Sensitivitaet: 0.56 ng/mL
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Immune molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-GM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-GM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human Anti-GM1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0676