Assay Type: Sandwich. Assay length: 1.5h. Research Area: Cytokine,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFN-gamma, and the Human IFN-gamma standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IFN-gamma. After TMB substrate solution is added, only those wells that contain Human IFN-gamma and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human IFN-gamma in the samples is then determined by comparing the OD of the samples to the standard curve
ELK067ES
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