Rat cAMP(Cyclic Adenosine Monophosphate) ELISA Kit

Produktübersicht

• Artikelname: Rat cAMP(Cyclic Adenosine Monophosphate) ELISA Kit
• Artikelnummer: EBT-ELK0754
• Hersteller Artikelnummer: ELK0754
• Alternativnummer: EBT-ELK0754-96, EBT-ELK0754-48, EBT-ELK0754-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Rat
• Alternative Synonym: c-AMP, 3-5-Cyclic Adenosine Monophosphate, Adenosine Cyclophosphate

Produkteigenschaften

• Konzentration: 20000 pg/mL
• Detektionsbereich: 312.5-20000 pg/mL
• Sensitivitaet: 143.3 pg/mL
• Proben: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Competitive Inhibition. Assay length: 2.5h. Research Area: Signal transduction,Metabolic pathway,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat cAMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat cAMP in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK0754