Human IFIT-1(Interferon-induced protein with tetratricopeptide repeats 1) ELISA Kit

Produktübersicht

• Artikelname: Human IFIT-1(Interferon-induced protein with tetratricopeptide repeats 1) ELISA Kit
• Artikelnummer: EBT-ELK0759
• Hersteller Artikelnummer: ELK0759
• Alternativnummer: EBT-ELK0759-96,EBT-ELK0759-48,EBT-ELK0759-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human
• Alternative Synonym: Interferon-induced 56 kDa protein (IFI-56K, P56)

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.069 ng/mL
• Proben: Tissue homogenates, cell lysates and other biological fluids.

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFIT-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFIT-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFIT-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human IFIT-1 in the samples is then determined by comparing the OD of the samples to the standard curve