EasyStep Human IL5(Interleukin 5) ELISA Kit

Artikelnummer: EBT-ELK080ES
Artikelname: EasyStep Human IL5(Interleukin 5) ELISA Kit
Artikelnummer: EBT-ELK080ES
Hersteller Artikelnummer: ELK080ES
Alternativnummer: EBT-ELK080ES-96, EBT-ELK080ES-48, EBT-ELK080ES-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: IL-5, E-CSF, ESGF, EDF, TRF, Colony-Stimulating Factor,Eosinophil, T-Cell replacing Factor, B Cell Differentiation Factor I, Eosinophil Differentiation Factor
Konzentration: 200 pg/mL
Detektionsbereich: 3.13-200 pg/mL
Sensitivitaet: 0.15 pg/mL
Proben: Serum, plasma, cell culture supernatants
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 1.5h. Research Area: Cytokine,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL5, and the Human IL5 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL5. After TMB substrate solution is added, only those wells that contain Human IL5 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human IL5 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK080ES