Rat hs-cTnT(High Sensitivity Cardiac Troponin T) Microsample ELISA Kit

Produktübersicht

• Artikelname: Rat hs-cTnT(High Sensitivity Cardiac Troponin T) Microsample ELISA Kit
• Artikelnummer: EBT-ELK0844MS
• Hersteller Artikelnummer: ELK0844MS
• Alternativnummer: EBT-ELK0844MS-96, EBT-ELK0844MS-48, EBT-ELK0844MS-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Rat
• Alternative Synonym: High Sensitivity Cardiac Troponin T

Produkteigenschaften

• Konzentration: 1000 pg/mL
• Detektionsbereich: 15.63-1000 pg/mL
• Sensitivitaet: 5.3 pg/mL
• Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cardiovascular biology. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-cTnT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-cTnT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-cTnT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat hs-cTnT in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK0844MS