Human IA-2 Ab(Insulinoma associated antigen 2 Autoantibody) ELISA Kit

Produktübersicht

• Artikelname: Human IA-2 Ab(Insulinoma associated antigen 2 Autoantibody) ELISA Kit
• Artikelnummer: EBT-ELK0858
• Hersteller Artikelnummer: ELK0858
• Alternativnummer: EBT-ELK0858-96, EBT-ELK0858-48, EBT-ELK0858-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human
• Alternative Synonym: IA2 Ab

Produkteigenschaften

• Konzentration: 4000 U/mL
• Detektionsbereich: 62.5-4000 U/mL
• Sensitivitaet: 26.8 U/mL
• Proben: tissue homogenates, cell lysates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IA-2 Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IA-2 Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IA-2 Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human IA-2 Ab in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK0858