Mouse PADI6(Peptidyl Arginine Deiminase Type VI) ELISA Kit

Artikelnummer: EBT-ELK0886
Artikelname: Mouse PADI6(Peptidyl Arginine Deiminase Type VI) ELISA Kit
Artikelnummer: EBT-ELK0886
Hersteller Artikelnummer: ELK0886
Alternativnummer: EBT-ELK0886-96, EBT-ELK0886-48, EBT-ELK0886-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: Peptidylarginine deiminase VI, Peptidyl arginine deiminase-like protein
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.073 ng/mL
UniProt: Q8K3V4
Proben: Tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PADI6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PADI6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PADI6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse PADI6 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0886