Hamster TNF-a(Tumor Necrosis Factor-alpha) ELISA Kit

Produktübersicht

• Artikelname: Hamster TNF-a(Tumor Necrosis Factor-alpha) ELISA Kit
• Artikelnummer: EBT-ELK0928
• Hersteller Artikelnummer: ELK0928
• Alternativnummer: EBT-ELK0928-96, EBT-ELK0928-48, EBT-ELK0928-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Hamster
• Alternative Synonym: DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2

Produkteigenschaften

• Konzentration: 200 pg/mL
• Detektionsbereich: 3.13-200 pg/mL
• Sensitivitaet: 1.09 pg/mL
• Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster TNF-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster TNF-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster TNF-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Hamster TNF-a in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK0928