Rat p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit

Produktübersicht

• Artikelname: Rat p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit
• Artikelnummer: EBT-ELK0962
• Hersteller Artikelnummer: ELK0962
• Alternativnummer: EBT-ELK0962-96, EBT-ELK0962-48, EBT-ELK0962-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Rat
• Alternative Synonym: p-PRKAA1, p-AMPK, p-AMPKa1, p-AMPK alpha 1

Produkteigenschaften

• Konzentration: 200 ng/mL
• Detektionsbereich: 3.13-200 ng/mL
• Sensitivitaet: 1.25 ng/mL
• Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK0962