Sheep ADIPOQ(Adiponectin) ELISA Kit

Artikelnummer: EBT-ELK0997
Artikelname: Sheep ADIPOQ(Adiponectin) ELISA Kit
Artikelnummer: EBT-ELK0997
Hersteller Artikelnummer: ELK0997
Alternativnummer: EBT-ELK0997-96, EBT-ELK0997-48, EBT-ELK0997-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Sheep
Alternative Synonym: ADP, GBP28, ApM1, AdipoQ, Acrp30, ACDC, APM1, C1Q And Collagen Domain Containing, Adipocyte Complement-Related Protein Of 30 KDa, Adipose Most Abundant Gene Transcript 1
Konzentration: 200 ng/mL
Detektionsbereich: 3.13-200 ng/mL
Sensitivitaet: 1.2 ng/mL
Proben: Serum, plasma and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,Infection immunity,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep ADIPOQ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep ADIPOQ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep ADIPOQ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Sheep ADIPOQ in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0997