Rat CXCR4(Chemokine C-X-C-Motif Receptor 4) Microsample ELISA Kit

Artikelnummer: EBT-ELK10056MS
Artikelname: Rat CXCR4(Chemokine C-X-C-Motif Receptor 4) Microsample ELISA Kit
Artikelnummer: EBT-ELK10056MS
Hersteller Artikelnummer: ELK10056MS
Alternativnummer: EBT-ELK10056MS-96, EBT-ELK10056MS-48, EBT-ELK10056MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: CD184, Fusin, NPYR, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYRL, WHIM, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.097 ng/mL
UniProt: O08565
Proben: tissue homogenates, cell lysates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,CD & Adhesion molecule,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CXCR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CXCR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CXCR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat CXCR4 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10056MS