Pig OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit

Artikelnummer: EBT-ELK10059
Artikelname: Pig OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit
Artikelnummer: EBT-ELK10059
Hersteller Artikelnummer: ELK10059
Alternativnummer: EBT-ELK10059-96, EBT-ELK10059-48, EBT-ELK10059-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Porcine
Alternative Synonym: OL
Konzentration: 100 ng/mL
Detektionsbereich: 1.57-100 ng/mL
Sensitivitaet: 0.56 ng/mL
Proben: serum, plasma and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,Endocrinology,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig OxLDL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig OxLDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig OxLDL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Pig OxLDL in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10059