Cattle AGC(Aggrecan) ELISA Kit

Artikelnummer: EBT-ELK10149
Artikelname: Cattle AGC(Aggrecan) ELISA Kit
Artikelnummer: EBT-ELK10149
Hersteller Artikelnummer: ELK10149
Alternativnummer: EBT-ELK10149-96, EBT-ELK10149-48, EBT-ELK10149-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Bovine
Alternative Synonym: ACAN, AGC1, AGCAN, CSPG1, CSPCP, CSPGCP, MSK16, SEDK, Cartilage-specific proteoglycan core protein, Large Aggregating Proteoglycan, Chondroitin Sulfate Proteoglycan 1
Konzentration: 20 µg/mL
Detektionsbereich: 0.32-20 µg/mL
Sensitivitaet: 0.121 µg/mL
Proben: Serum, plasma and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Metabolic pathway,Bone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle AGC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle AGC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle AGC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cattle AGC in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10149