Mouse SELE(Selectin, Endothelium) ELISA Kit

Produktübersicht

• Artikelname: Mouse SELE(Selectin, Endothelium) ELISA Kit
• Artikelnummer: EBT-ELK10176
• Hersteller Artikelnummer: ELK10176
• Alternativnummer: EBT-ELK10176-96, EBT-ELK10176-48, EBT-ELK10176-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Mouse
• Alternative Synonym: CD62E, CD62-E, E-Selectin, ELAM, ELAM1, ESEL, SEL-E, E-LAM, E-LAM1, E-SEL, LECAM2, Endothelial Leukocyte Adhesion Molecule 1, CD62 Antigen-Like Family Member E

Produkteigenschaften

• Konzentration: 50 ng/mL
• Detektionsbereich: 0.78-50 ng/mL
• Sensitivitaet: 0.18 ng/mL
• UniProt: Q00690
• Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,CD & Adhesion molecule,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SELE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SELE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SELE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse SELE in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK10176