Rat hs-CRP(High Sensitivity C Reactive Protein) ELISA Kit

Produktübersicht

• Artikelname: Rat hs-CRP(High Sensitivity C Reactive Protein) ELISA Kit
• Artikelnummer: EBT-ELK10185
• Hersteller Artikelnummer: ELK10185
• Alternativnummer: EBT-ELK10185-96, EBT-ELK10185-48, EBT-ELK10185-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Rat
• Alternative Synonym: C-RP, PTX1, Pentraxin-Related, Pentraxin 1

Produkteigenschaften

• Konzentration: 50 µg/mL
• Detektionsbereich: 0.78-50 µg/mL
• Sensitivitaet: 0.16 µg/mL
• UniProt: P48199
• Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,Infection immunity,Cardiovascular biology,Rheumatology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK10185