Human S100A11(S100 Calcium Binding Protein A11) Microsample ELISA Kit

Artikelnummer: EBT-ELK10242MS
Artikelname: Human S100A11(S100 Calcium Binding Protein A11) Microsample ELISA Kit
Artikelnummer: EBT-ELK10242MS
Hersteller Artikelnummer: ELK10242MS
Alternativnummer: EBT-ELK10242MS-96, EBT-ELK10242MS-48, EBT-ELK10242MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: S100-A11, S100C, MLN70, Protein S100-C, Calgizzarin, Metastatic lymph node gene 70 protein, Protein S100-A11, N-terminally processed
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.06 ng/mL
UniProt: P31949
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,Infection immunity,Developmental science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S100A11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100A11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S100A11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human S100A11 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10242MS