Mouse DPP4(Dipeptidyl Peptidase IV) ELISA Kit

Artikelnummer: EBT-ELK10301
Artikelname: Mouse DPP4(Dipeptidyl Peptidase IV) ELISA Kit
Artikelnummer: EBT-ELK10301
Hersteller Artikelnummer: ELK10301
Alternativnummer: EBT-ELK10301-96, EBT-ELK10301-48, EBT-ELK10301-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: CD26, ADA, DPPIV, DPP-IV, ADABP, ADCP2, TP103, Adenosine Deaminase Complexing Protein 2, T-cell activation antigen CD26
Konzentration: 40 ng/mL
Detektionsbereich: 0.63-40 ng/mL
Sensitivitaet: 0.21 ng/mL
UniProt: P28843
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Enzyme & Kinase,Apoptosis,Tumor immunity,Endocrinology,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DPP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DPP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DPP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse DPP4 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10301