Mouse IGF2(Insulin Like Growth Factor 2) ELISA Kit

Produktübersicht

• Artikelname: Mouse IGF2(Insulin Like Growth Factor 2) ELISA Kit
• Artikelnummer: EBT-ELK10390
• Hersteller Artikelnummer: ELK10390
• Alternativnummer: EBT-ELK10390-96, EBT-ELK10390-48, EBT-ELK10390-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Mouse
• Alternative Synonym: INSIGF, IGF-II, Somatomedin A, Preptin, Insulin-like growth factor II Ala-25 Del

Produkteigenschaften

• Konzentration: 40 ng/mL
• Detektionsbereich: 0.63-40 ng/mL
• Sensitivitaet: 0.221 ng/mL
• UniProt: P09535
• Proben: Serum, plasma and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,Endocrinology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IGF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse IGF2 in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK10390