Rabbit COL1a2(Collagen Type I Alpha 2) ELISA Kit

Artikelnummer: EBT-ELK10401
Artikelname: Rabbit COL1a2(Collagen Type I Alpha 2) ELISA Kit
Artikelnummer: EBT-ELK10401
Hersteller Artikelnummer: ELK10401
Alternativnummer: EBT-ELK10401-96, EBT-ELK10401-48, EBT-ELK10401-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rabbit
Alternative Synonym: COL1-A2, OI4, Collagen Alpha-2(I)chain, osteogenesis imperfecta type IV, Collagen Of Skin,Tendon And bone,Alpha-2 Chain
Konzentration: 50 ng/mL
Detektionsbereich: 0.78-50 ng/mL
Sensitivitaet: 0.16 ng/mL
UniProt: Q28668
Proben: Serum, plasma and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,Bone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit COL1a2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit COL1a2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit COL1a2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rabbit COL1a2 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10401