Human APOH(Apolipoprotein H) ELISA Kit

Artikelnummer: EBT-ELK10403
Artikelname: Human APOH(Apolipoprotein H) ELISA Kit
Artikelnummer: EBT-ELK10403
Hersteller Artikelnummer: ELK10403
Alternativnummer: EBT-ELK10403-96, EBT-ELK10403-48, EBT-ELK10403-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: Apo-H, B2G1, BG, B2-GP1, B2GP1, Previously, Beta 2 Glycoprotein 1, APC inhibitor, Activated protein C-binding protein, Anticardiolipin cofactor, Beta(2)GPI
Konzentration: 400 ng/mL
Detektionsbereich: 6.25-400 ng/mL
Sensitivitaet: 2.71 ng/mL
UniProt: P02749
Proben: Serum, plasma and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human APOH in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10403