Human NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

Produktübersicht

• Artikelname: Human NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit
• Artikelnummer: EBT-ELK10409
• Hersteller Artikelnummer: ELK10409
• Alternativnummer: EBT-ELK10409-96, EBT-ELK10409-48, EBT-ELK10409-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human
• Alternative Synonym: NT-Pro-ANP, N-ANP

Produkteigenschaften

• Konzentration: 50 ng/mL
• Detektionsbereich: 0.79-50 ng/mL
• Sensitivitaet: 0.31 ng/mL
• Proben: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Endocrinology,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK10409