Human MASP2(Mannose Associated Serine Protease 2) Microsample ELISA Kit

Artikelnummer: EBT-ELK10523MS
Artikelname: Human MASP2(Mannose Associated Serine Protease 2) Microsample ELISA Kit
Artikelnummer: EBT-ELK10523MS
Hersteller Artikelnummer: ELK10523MS
Alternativnummer: EBT-ELK10523MS-96, EBT-ELK10523MS-48, EBT-ELK10523MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: Mannose-binding protein-associated serine protease 2, mannan-Binding Lectin Serine Peptidase 2, MBL-associated serine protease 2
Konzentration: 100 ng/mL
Detektionsbereich: 1.56-100 ng/mL
Sensitivitaet: 0.63 ng/mL
UniProt: O00187
Proben: serum, plasma and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,Infection immunity,Immune molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MASP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MASP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MASP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human MASP2 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10523MS