Mouse IGFBP7(Insulin Like Growth Factor Binding Protein 7) ELISA Kit

Artikelnummer: EBT-ELK10566
Artikelname: Mouse IGFBP7(Insulin Like Growth Factor Binding Protein 7) ELISA Kit
Artikelnummer: EBT-ELK10566
Hersteller Artikelnummer: ELK10566
Alternativnummer: EBT-ELK10566-96, EBT-ELK10566-48, EBT-ELK10566-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: FSTL2, IGFBP-7v, MAC25, PSF, TAF, IGFBP-rP1, MAC25 protein, Prostacyclin-stimulating factor, PGI2-stimulating factor, Tumor-derived adhesion factor
Konzentration: 100 ng/mL
Detektionsbereich: 1.56-100 ng/mL
Sensitivitaet: 0.51 ng/mL
UniProt: Q61581
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IGFBP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IGFBP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse IGFBP7 in the samples is then determined by comparing the OD of the samples to the standard curve
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