Human PCSK9(Proprotein Convertase Subtilisin/Kexin Type 9) ELISA Kit

Artikelnummer: EBT-ELK10569
Artikelname: Human PCSK9(Proprotein Convertase Subtilisin/Kexin Type 9) ELISA Kit
Artikelnummer: EBT-ELK10569
Hersteller Artikelnummer: ELK10569
Alternativnummer: EBT-ELK10569-96, EBT-ELK10569-48, EBT-ELK10569-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: FH3, HCHOLA3, NARC1, Hypercholesterolemia,Autosomal Dominant 3, Neural apoptosis-regulated convertase 1, Proprotein convertase 9, Subtilisin/kexin-like protease PC9
Konzentration: 100 ng/mL
Detektionsbereich: 1.57-100 ng/mL
Sensitivitaet: 0.49 ng/mL
UniProt: Q8NBP7
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PCSK9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PCSK9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PCSK9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PCSK9 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10569