Mouse HCAM(Homing Associated Cell Adhesion Molecule) Microsample ELISA Kit

Artikelnummer: EBT-ELK10575MS
Artikelname: Mouse HCAM(Homing Associated Cell Adhesion Molecule) Microsample ELISA Kit
Artikelnummer: EBT-ELK10575MS
Hersteller Artikelnummer: ELK10575MS
Alternativnummer: EBT-ELK10575MS-96, EBT-ELK10575MS-48, EBT-ELK10575MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: CDW44, CD44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH-I, Pgp1, Hematopoietic Cell E- And L-Selectin Ligand, Chondroitin Sulfate Proteoglycan 8
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.061 ng/mL
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,CD & Adhesion molecule,Tumor immunity,Infection immunity,Immune molecule,Hematology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse HCAM in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10575MS