Mouse MSP(Macrophage Stimulating Protein) ELISA Kit

Artikelnummer: EBT-ELK10599
Artikelname: Mouse MSP(Macrophage Stimulating Protein) ELISA Kit
Artikelnummer: EBT-ELK10599
Hersteller Artikelnummer: ELK10599
Alternativnummer: EBT-ELK10599-96, EBT-ELK10599-48, EBT-ELK10599-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: MST1, HGFL, NF15S2, Hepatocyte Growth Factor-Like Protein, Macrophage Stimulating 1
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.054 ng/mL
Proben: Serum, plasma and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Tumor immunity,Infection immunity,Hepatology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MSP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse MSP in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10599