Pig IGFBP1(Insulin Like Growth Factor Binding Protein 1) ELISA Kit

Artikelnummer: EBT-ELK10680
Artikelname: Pig IGFBP1(Insulin Like Growth Factor Binding Protein 1) ELISA Kit
Artikelnummer: EBT-ELK10680
Hersteller Artikelnummer: ELK10680
Alternativnummer: EBT-ELK10680-96, EBT-ELK10680-96X5, EBT-ELK10680-48
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Porcine
Alternative Synonym: IBP1, AFBP, IGF-BP25, PP12, Placental Protein 12, Amniotic Fluid Binding Protein, Alpha-Pregnancy-Associated Endometrial Globulin, Growth Hormone Independent-Binding Protein
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.057 ng/mL
UniProt: Q75ZP3
Proben: Serum, plasma and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,Endocrinology,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IGFBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IGFBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IGFBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Pig IGFBP1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10680