Cattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit

Artikelnummer: EBT-ELK10693
Artikelname: Cattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit
Artikelnummer: EBT-ELK10693
Hersteller Artikelnummer: ELK10693
Alternativnummer: EBT-ELK10693-96, EBT-ELK10693-96X5, EBT-ELK10693-48
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Bovine
Alternative Synonym: BP4, HT29-IGFBP, IBP4
Konzentration: 100 ng/mL
Detektionsbereich: 1.57-100 ng/mL
Sensitivitaet: 0.56 ng/mL
UniProt: Q05716
Proben: Serum, plasma and other biological fluids
Anwendungsbeschreibung: Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Apoptosis,Tumor immunity,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle IGFBP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGFBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cattle IGFBP4 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10693