Guinea pig IGF1(Insulin Like Growth Factor 1) ELISA Kit

Artikelnummer: EBT-ELK10697
Artikelname: Guinea pig IGF1(Insulin Like Growth Factor 1) ELISA Kit
Artikelnummer: EBT-ELK10697
Hersteller Artikelnummer: ELK10697
Alternativnummer: EBT-ELK10697-96, EBT-ELK10697-96X5, EBT-ELK10697-48
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Guinea pig
Alternative Synonym: IGFI, IGF1A, IBP1, MGF, Somatomedin C, Mechano Growth Gactor
Konzentration: 1000 ng/mL
Detektionsbereich: 15.63-1000 ng/mL
Sensitivitaet: 6.3 ng/mL
UniProt: P17647
Proben: Serum, plasma and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,Endocrinology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig IGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig IGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig IGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Guinea pig IGF1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10697