Rabbit AGC(Aggrecan) ELISA Kit

Artikelnummer: EBT-ELK10719
Artikelname: Rabbit AGC(Aggrecan) ELISA Kit
Artikelnummer: EBT-ELK10719
Hersteller Artikelnummer: ELK10719
Alternativnummer: EBT-ELK10719-96, EBT-ELK10719-96X5, EBT-ELK10719-48
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rabbit
Alternative Synonym: ACAN, AGC1, AGCAN, CSPG1, CSPCP, CSPGCP, MSK16, SEDK, Cartilage-specific proteoglycan core protein, Large Aggregating Proteoglycan, Chondroitin Sulfate Proteoglycan 1
Konzentration: 10 µg/mL
Detektionsbereich: 0.16-10 µg/mL
Sensitivitaet: 0.052 µg/mL
UniProt: Q28670
Proben: Serum, plasma and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Metabolic pathway,Bone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit AGC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit AGC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit AGC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rabbit AGC in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10719