Pig LTF(Lactoferrin) ELISA Kit

Artikelnummer: EBT-ELK10732
Artikelname: Pig LTF(Lactoferrin) ELISA Kit
Artikelnummer: EBT-ELK10732
Hersteller Artikelnummer: ELK10732
Alternativnummer: EBT-ELK10732-96, EBT-ELK10732-96X5, EBT-ELK10732-48
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Porcine
Alternative Synonym: HLF2, LF, GIG12, Lactotransferrin, Growth-inhibiting protein 12, Talalactoferrin, Lfcin-H, Kaliocin-1
Konzentration: 5000 ng/mL
Detektionsbereich: 78.13-5000 ng/mL
Sensitivitaet: 19.3 ng/mL
UniProt: P14632
Proben: Serum, plasma and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig LTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig LTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig LTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Pig LTF in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10732