Rat KNG1(Kininogen 1) ELISA Kit

Artikelnummer: EBT-ELK10753
Artikelname: Rat KNG1(Kininogen 1) ELISA Kit
Artikelnummer: EBT-ELK10753
Hersteller Artikelnummer: ELK10753
Alternativnummer: EBT-ELK10753-96, EBT-ELK10753-96X5, EBT-ELK10753-48
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: BDK, KNG, HMWK, Alpha-2-Thiol Proteinase Inhibitor, Bradykinin, Kallidin, Fitzgerald factor, High molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, Ile-Ser-Bradykinin
Konzentration: 100 µg/mL
Detektionsbereich: 1.57-100 µg/mL
Sensitivitaet: 0.67 µg/mL
UniProt: P08934
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Hematology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KNG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KNG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KNG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat KNG1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10753