Rat STAT6(Signal Transducer And Activator Of Transcription 6) ELISA Kit

Artikelnummer: EBT-ELK10787
Artikelname: Rat STAT6(Signal Transducer And Activator Of Transcription 6) ELISA Kit
Artikelnummer: EBT-ELK10787
Hersteller Artikelnummer: ELK10787
Alternativnummer: EBT-ELK10787-96, EBT-ELK10787-96X5, EBT-ELK10787-48
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: IL-4-STAT, STAT6B, STAT6C, Signal Transducer And Activator Of Transcription 6,Interleukin-4 Induced
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.057 ng/mL
UniProt: O70429
Proben: Serum, plasma, tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat STAT6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat STAT6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat STAT6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat STAT6 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10787