Horse SAA(Serum Amyloid A) ELISA Kit

Artikelnummer: EBT-ELK10876
Artikelname: Horse SAA(Serum Amyloid A) ELISA Kit
Artikelnummer: EBT-ELK10876
Hersteller Artikelnummer: ELK10876
Alternativnummer: EBT-ELK10876-96, EBT-ELK10876-96X5, EBT-ELK10876-48
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Equine
Alternative Synonym: SA-A, SAA1, PIG4, TP53I4, Amyloid protein A, Amyloid fibril protein AA, Serum amyloid A-1 protein
Konzentration: 200 ng/mL
Detektionsbereich: 3.13-200 ng/mL
Sensitivitaet: 0.273 ng/mL
UniProt: Q6X9W5
Proben: serum, plasma and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Infection immunity,Kidney biomarker,Hepatology,Urology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse SAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse SAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse SAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Horse SAA in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10876