Mouse Lptn/LTN/XCL1 (Lymphotactin) Microsample ELISA Kit

Produktübersicht

• Artikelname: Mouse Lptn/LTN/XCL1 (Lymphotactin) Microsample ELISA Kit
• Artikelnummer: EBT-ELK10951MS
• Hersteller Artikelnummer: ELK10951MS
• Alternativnummer: EBT-ELK10951MS-96, EBT-ELK10951MS-48, EBT-ELK10951MS-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Mouse
• Alternative Synonym: Xcl1 , Lptn , Ltn , Scyc1 , Lymphotactin , C motif chemokine 1 , Cytokine SCM-1 , Lymphotaxin , Small-inducible cytokine C1

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.064 ng/mL
• UniProt: P47993
• Proben: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Tumor immunity,Infection immunity,Dermatology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Lptn/LTN/XCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Lptn/LTN/XCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Lptn/LTN/XCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse Lptn/LTN/XCL1 in the samples is then determined by comparing the OD of the samples to the standard curve