Human sTFR(Soluble Transferrin Receptor) ELISA Kit

Artikelnummer: EBT-ELK10988
Artikelname: Human sTFR(Soluble Transferrin Receptor) ELISA Kit
Artikelnummer: EBT-ELK10988
Hersteller Artikelnummer: ELK10988
Alternativnummer: EBT-ELK10988-96, EBT-ELK10988-96X5, EBT-ELK10988-48
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CD71, TFR1, P90, TFRC, T9, Trfr, sTfR, Transferrin receptor protein 1, serum form
Konzentration: 300 ng/mL
Detektionsbereich: 4.69-300 ng/mL
Sensitivitaet: 1.57 ng/mL
Proben: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,CD & Adhesion molecule,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sTFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sTFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human sTFR in the samples is then determined by comparing the OD of the samples to the standard curve
ELK10988