Rat TFR(Transferrin Receptor) ELISA Kit

Artikelnummer: EBT-ELK11162
Artikelname: Rat TFR(Transferrin Receptor) ELISA Kit
Artikelnummer: EBT-ELK11162
Hersteller Artikelnummer: ELK11162
Alternativnummer: EBT-ELK11162-96, EBT-ELK11162-48, EBT-ELK11162-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: CD71, TFR1, P90, TFRC, T9, Trfr, sTfR, Transferrin receptor protein 1, serum form
Konzentration: 10 µg/mL
Detektionsbereich: 0.16-10 µg/mL
Sensitivitaet: 0.053 µg/mL
UniProt: Q99376
Proben: Serum, plasma and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,CD & Adhesion molecule,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat TFR in the samples is then determined by comparing the OD of the samples to the standard curve
ELK11162