Human BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit

Artikelnummer: EBT-ELK1200
Artikelname: Human BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit
Artikelnummer: EBT-ELK1200
Hersteller Artikelnummer: ELK1200
Alternativnummer: EBT-ELK1200-96, EBT-ELK1200-96X5, EBT-ELK1200-48
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: Beta 2M, BMG, B2MG, b2M, Beta-2-Microglobulin
Konzentration: 25 ng/mL
Detektionsbereich: 0.4-25 ng/mL
Sensitivitaet: 0.16 ng/mL
UniProt: P61769
Proben: serum, plasma, urine and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Infection immunity,Kidney biomarker,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1200