Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Artikelnummer: EBT-ELK1212
Artikelname: Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Artikelnummer: EBT-ELK1212
Hersteller Artikelnummer: ELK1212
Alternativnummer: EBT-ELK1212-96, EBT-ELK1212-48, EBT-ELK1212-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: NT-Pro-BNP, , N-BNP, ANFB_HUMAN, BNP(1-32), BNP(5-29), BNP-32, Gamma-brain natriuretic peptide, nppb
Konzentration: 2500 pg/mL
Detektionsbereich: 39.07-2500 pg/mL
Sensitivitaet: 15 pg/mL
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Endocrinology,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1212