Mouse GROa/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit

Artikelnummer: EBT-ELK1238
Artikelname: Mouse GROa/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit
Artikelnummer: EBT-ELK1238
Hersteller Artikelnummer: ELK1238
Alternativnummer: EBT-ELK1238-96, EBT-ELK1238-48, EBT-ELK1238-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: CXCL1, GRO1, GROa, GRO-A, MGSA-A, NAP3, SCYB1, Chemokine C-X-C-Motif Ligand 1, Melanoma Growth Stimulating Activity Alpha, Fibroblast Secretory Protein, Neutrophil Activating Protein 3
Konzentration: 1000 pg/mL
Detektionsbereich: 15.63-1000 pg/mL
Sensitivitaet: 6 pg/mL
UniProt: P12850
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GROa/CXCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GROa/CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse GROa/CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1238