Mouse NF-kappaB p105(Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit

Produktübersicht

• Artikelname: Mouse NF-kappaB p105(Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit
• Artikelnummer: EBT-ELK1337MS
• Hersteller Artikelnummer: ELK1337MS
• Alternativnummer: EBT-ELK1337MS-96, EBT-ELK1337MS-48, EBT-ELK1337MS-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Mouse
• Alternative Synonym: NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit, NFkB, Nuclear Factor Kappa B

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.06 ng/mL
• UniProt: P25799
• Proben: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NF-kappaBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NF-kappaBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NF-kappaBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse NF-kappaBp105 in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK1337MS