Human ITaC(Interferon Inducible T-Cell Alpha Chemoattractant) ELISA Kit

Artikelnummer: EBT-ELK1344
Artikelname: Human ITaC(Interferon Inducible T-Cell Alpha Chemoattractant) ELISA Kit
Artikelnummer: EBT-ELK1344
Hersteller Artikelnummer: ELK1344
Alternativnummer: EBT-ELK1344-96, EBT-ELK1344-48, EBT-ELK1344-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CXCL11, I-TAC, IP9, SCYB11, SCYB9B, B-R1, Chemokine(C-X-C-Motif)ligand 11, Small Inducible Cytokine Subfamily B Member 11, Interferon gamma-inducible protein 9
Konzentration: 4000 pg/mL
Detektionsbereich: 62.5-4000 pg/mL
Sensitivitaet: 23 pg/mL
UniProt: O14625
Proben: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Signal transduction,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITaC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITaC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITaC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human ITaC in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1344